MARC details
| 000 -LIDER |
|---|
| fixed length control field |
03728nam a22004935i 4500 |
| 001 - CONTROL NUMBER |
|---|
| control field |
978-3-319-45295-1 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
DE-He213 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20180206183007.0 |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
|---|
| fixed length control field |
cr nn 008mamaa |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
161111s2016 gw | s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
9783319452951 |
| -- |
978-3-319-45295-1 |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER |
|---|
| Classification number |
QR180-189.5 |
| 072 #7 - SUBJECT CATEGORY CODE |
|---|
| Subject category code |
MJCM |
| Source |
bicssc |
| 072 #7 - SUBJECT CATEGORY CODE |
|---|
| Subject category code |
MED044000 |
| Source |
bisacsh |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
616.079 |
| Edition number |
23 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Janetzki, Sylvia. |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Elispot for Rookies (and Experts Too) |
| Medium |
[recurso electrónico] / |
| Statement of responsibility, etc. |
by Sylvia Janetzki. |
| 264 #1 - |
|---|
| -- |
Cham : |
| -- |
Springer International Publishing : |
| -- |
Imprint: Springer, |
| -- |
2016. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
VIII, 93 p. 39 illus., 32 illus. in color. |
| Other physical details |
online resource. |
| 336 ## - |
|---|
| -- |
text |
| -- |
txt |
| -- |
rdacontent |
| 337 ## - |
|---|
| -- |
computer |
| -- |
c |
| -- |
rdamedia |
| 338 ## - |
|---|
| -- |
online resource |
| -- |
cr |
| -- |
rdacarrier |
| 347 ## - |
|---|
| -- |
text file |
| -- |
PDF |
| -- |
rda |
| 490 1# - SERIES STATEMENT |
|---|
| Series statement |
Techniques in Life Science and Biomedicine for the Non-Expert, |
| International Standard Serial Number |
2367-1114 |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Preface -- Overview -- Historic Overview -- Principles of Elispot -- Modifications -- Simplifications -- Coating Step -- Detection Step -- Adaptations -- Detection of specific antibodies -- Detection of multiple analytes in one well -- Dual color Elispot -- Fluorospot -- Summary -- Important Assay Details -- Reagents and Materials -- Elispot plates -- Antibody choices -- Enzymatic conjugates and substrates -- Fluorescent dyes -- Culture media -- Blocking buffers -- Washing buffers -- Important laboratory supplies -- Pipettes -- Filters -- Other supplies -- Automated Elispot reader -- Sample preparation -- Overview -- Whole blood and PBMC -- Blood draw and anticoagulants -- PBMC isolation -- Time frame between blood draw and PBMC isolation -- Immune cells obtained from tissue -- Freezing of cells -- Thawing of cells -- Cell counting and viability -- Overnight resting -- Isolation of sub-populations of cells -- Expansion of cells -- Planning the experiment -- Controls -- Negative controls -- Positive controls -- External controls -- Plate layout -- Timing -- The Elispot assay -- Coating -- Blocking -- Effector cell preparation -- Stimulant preparation -- Peptides and peptide pools -- Proteins and lysate -- Antigen presenters -- Control stimulants -- Reference samples -- Plating out cells and stimulants -- Incubation -- Removal of cells -- Spot development -- Enumeration of spots -- Data analysis -- Quality control -- Reporting guidelines -- Troubleshooting -- References. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author´s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Término temático o nombre geográfico como elemento de entrada |
Medicine. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Término temático o nombre geográfico como elemento de entrada |
Immunology. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Término temático o nombre geográfico como elemento de entrada |
Laboratory medicine. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Término temático o nombre geográfico como elemento de entrada |
Cell biology. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Término temático o nombre geográfico como elemento de entrada |
Biomedicine. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Término temático o nombre geográfico como elemento de entrada |
Immunology. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Término temático o nombre geográfico como elemento de entrada |
Cell Biology. |
| 650 24 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Término temático o nombre geográfico como elemento de entrada |
Laboratory Medicine. |
| 710 2# - ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
SpringerLink (Online service) |
| 773 0# - HOST ITEM ENTRY |
|---|
| Title |
Springer eBooks |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Relationship information |
Printed edition: |
| International Standard Book Number |
9783319452937 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
|---|
| Uniform title |
Techniques in Life Science and Biomedicine for the Non-Expert, |
| -- |
2367-1114 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Public note |
Libro electrónico |
| Uniform Resource Identifier |
http://148.231.10.114:2048/login?url=http://dx.doi.org/10.1007/978-3-319-45295-1 |
| 912 ## - |
|---|
| -- |
ZDB-2-SBL |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Libro Electrónico |
