Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications [recurso electrónico] / by Erika Pestana, Sandor Belak, Adama Diallo, John R. Crowther, Gerrit J. Viljoen.

Por: Pestana, Erika [author.]Colaborador(es): Belak, Sandor [author.] | Diallo, Adama [author.] | Crowther, John R [author.] | Viljoen, Gerrit J [author.] | SpringerLink (Online service)Tipo de material: TextoTextoEditor: Dordrecht : Springer Netherlands, 2010Descripción: XIII, 310 p. online resourceTipo de contenido: text Tipo de medio: computer Tipo de portador: online resourceISBN: 9789048131327Tema(s): Life sciences | Medicine | Medical virology | Emerging infectious diseases | Agriculture | Cytology -- Research_xMethodology | Life Sciences | Biological Techniques | Infectious Diseases | Virology | Molecular Medicine | Agriculture | Biomedicine generalFormatos físicos adicionales: Printed edition:: Sin títuloClasificación CDD: 570.28 Clasificación LoC:QH324Recursos en línea: Libro electrónicoTexto
Contenidos:
Background -- Traditional PCR -- Real-Time PCR – The Basic Principles -- New Trends in the Diagnosis and Molecular Epidemiology of Viral Diseases -- Disease Diagnosis Using Real-Time PCR Specific Procedures for Important Veterinary Pathogens -- PCR Laboratory Set-up -- Analysis and Troubleshooting -- Specifications for PCR Machines.
En: Springer eBooksResumen: This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.
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Tipo de ítem Biblioteca actual Colección Signatura Copia número Estado Fecha de vencimiento Código de barras
Libro Electrónico Biblioteca Electrónica
Colección de Libros Electrónicos QH324 (Browse shelf(Abre debajo)) 1 No para préstamo 377533-2001

Background -- Traditional PCR -- Real-Time PCR – The Basic Principles -- New Trends in the Diagnosis and Molecular Epidemiology of Viral Diseases -- Disease Diagnosis Using Real-Time PCR Specific Procedures for Important Veterinary Pathogens -- PCR Laboratory Set-up -- Analysis and Troubleshooting -- Specifications for PCR Machines.

This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.

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