Molecular biology techniques [recurso electrónico] : a classroom laboratory manual / Susan Carson, Heather Miller, D. Scott Witherow.
Tipo de material: TextoDetalles de publicación: Amsterdam ; Boston : Elsevier /Academic Press, 2012Edición: 3rd edDescripción: 1 online resource (xxvi, 200 pages) : color illustrationsTipo de contenido: text Tipo de medio: computer Tipo de portador: online resourceISBN: 9780123855459 (electronic bk.); 0123855454 (electronic bk.)Tema(s): Molecular biology -- Laboratory manuals | Molecular biology -- Technique -- Handbooks, manuals, etc | DNA, Recombinant -- Laboratory Manuals | Molecular Biology -- methods -- Laboratory Manuals | Protein Engineering -- Laboratory Manuals | SCIENCE -- Life Sciences -- Molecular Biology | Molecular biology | Molecular biology -- Technique | Methode | MolekularbiologieGénero/Forma: Electronic books. | Handbooks, manuals, etc.Formatos físicos adicionales: Print version:: Molecular biology techniques.Clasificación CDD: 572.8/078 Clasificación LoC:QH506 | .C36 2012ebRecursos en línea: Libro electrónico ScienceDirectTipo de ítem | Biblioteca actual | Colección | Signatura | Copia número | Estado | Fecha de vencimiento | Código de barras |
---|---|---|---|---|---|---|---|
Libro Electrónico | Biblioteca Electrónica | Colección de Libros Electrónicos | QH506 .C36 2012 EB (Browse shelf(Abre debajo)) | 1 | No para préstamo | 380634-2001 |
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QH506 Extracellular ATP and Adenosine as Regulators of Endothelial Cell Function | QH506 Microbial Biochemistry | QH506 .A45 2007 EB Fundamental molecular biology | QH506 .C36 2012 EB Molecular biology techniques | QH506 .M66 2011 Structural biology using electrons and X-rays | QH506 .R436 1999 EB Molecular biology techniques | QH509 Channels, carriers, and pumps : |
Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006.
Includes bibliographical references and index.
Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions.
Description based on print version record.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions.
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