Optical tweezers : methods and protocols / edited by Arne Gennerich

Colaborador(es): Gennerich, Arne [ed.]Tipo de material: TextoTextoSeries Detalles de publicación: New York : Humana Press, ©2017Descripción: xii, 555 p. : il. (algunas col.) ; 26 cmISBN: 9781493964192 (hbk. : alk. paper); 9781493964215Tema(s): Optical tweezers -- Laboratory manuals | Optical Tweezers | Biophysical Phenomena | Investigative Techniques | Pinzas ópticas -- Manuales de laboratorioClasificación LoC:TK8360.O69 | O68 2017
Contenidos:
Introduction to optical tweezers -- Exact theory of optical tweezers and its application to absolute calibration -- Beyond the Hookean spring model : direct measurement of optical forces through light momentum changes -- A surface-coupled optical trap with 1-bp precision via active stabilization -- Implementation and tuning of an optical tweezers force-clamp feedback system -- Custom-made microspheres for optical tweezers -- Optical torque wrench design and calibration -- High-resolution "fleezers" : dual-trap optical tweezers combined with single-molecule fluorescence detection -- Versatile quadruple-trap optical tweezers for dual DNA experiments -- Probing DNA-DNA interactions with a combination of quadruple-trap optical tweezers and microfluidics -- Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement -- Mechanically watching the ClpXP proteolytic machinery -- Deciphering the molecular mechanism of the bacteriophage [phi]29 DNA packaging motor -- Single-molecule protein folding experiments using high-precision optical tweezers -- Observing single RNA polymerase molecules down to base-pair resolution -- Optical tweezers-based measurements of forces and dynamics at microtubule ends -- Simultaneous manipulation and super-resolution fluorescence imaging of individual kinetochores coupled to microtubule tips -- Measurement of force-dependent release rates of cytoskeletal motors -- Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers -- Quantifying force and viscoelasticity inside living cells using an active-passive calibrated optical trap -- Measuring molecular forces using calibrated optical tweezers in living cells.
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"Published In: United States, 20 December 2016" --Distributor's website.

Incluye referencias bibliográficas e índice

Introduction to optical tweezers -- Exact theory of optical tweezers and its application to absolute calibration -- Beyond the Hookean spring model : direct measurement of optical forces through light momentum changes -- A surface-coupled optical trap with 1-bp precision via active stabilization -- Implementation and tuning of an optical tweezers force-clamp feedback system -- Custom-made microspheres for optical tweezers -- Optical torque wrench design and calibration -- High-resolution "fleezers" : dual-trap optical tweezers combined with single-molecule fluorescence detection -- Versatile quadruple-trap optical tweezers for dual DNA experiments -- Probing DNA-DNA interactions with a combination of quadruple-trap optical tweezers and microfluidics -- Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement -- Mechanically watching the ClpXP proteolytic machinery -- Deciphering the molecular mechanism of the bacteriophage [phi]29 DNA packaging motor -- Single-molecule protein folding experiments using high-precision optical tweezers -- Observing single RNA polymerase molecules down to base-pair resolution -- Optical tweezers-based measurements of forces and dynamics at microtubule ends -- Simultaneous manipulation and super-resolution fluorescence imaging of individual kinetochores coupled to microtubule tips -- Measurement of force-dependent release rates of cytoskeletal motors -- Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers -- Quantifying force and viscoelasticity inside living cells using an active-passive calibrated optical trap -- Measuring molecular forces using calibrated optical tweezers in living cells.

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