TY - BOOK AU - Ravid,Katya AU - Freshney,R.Ian TI - DNA transfer to cultured cells T2 - Culture of specialized cells SN - 0471165727 (pbk. alk. paper) AV - QH442.6 .D53 1998 PY - 1998/// CY - New York PB - Wiley-Liss KW - Ingeniería genética KW - Metodología KW - lemb KW - Transfección KW - Genetic engineering KW - Methodology KW - Transfection KW - Cell culture KW - Cells, Cultured KW - DNA KW - genetics N1 - Incluye referencias bibliográficas e índice; Ch. 1. Production and use of retroviruses-- Ch. 2. Baculovirus expression and the study of the regulation of the tumor suppressor protein p53-- Ch. 3. Electroporation of DNA into cultured cell lines-- Ch. 4. DNA transfer to blastula-derived cultures from ZebraFish-- Ch. 5. Transfection of rat adipose cells by electroporation-- Ch. 6. Calcium phosphate transfection-- Ch. 7. Optimization of calcium phosphate transfection for reporter gene analysis-- Ch. 8. Strontium phosphate transfection; Ch. 9. In vitro tissue transfection by calcium phosphate-- Ch. 10. DEAE-Dextran transfection-- Ch. 11. Cationic liposomes-- Ch. 12. Transfection of mammalian cells with yeast artificial chromosomes-- Ch. 13. Mapping human senescence genes using microcell-mediated chromosome transfer-- Ch. 14. Methotrexate and DHFR amplification UR - http://www.loc.gov/catdir/description/wiley037/97050456.html UR - http://www.loc.gov/catdir/toc/onix05/97050456.html ER -