TY  - BOOK
AU  - Hoy,Marjorie A.
TI  - Insect molecular genetics: an introduction to principles and applications 
SN  - 9780240821313 (electronic bk.)
AV  - QL493 .H69 2013
U1  - 595.7 23
PY  - 2013///
KW  - Insects
KW  - Molecular genetics
KW  - SCIENCE
KW  - Life Sciences
KW  - Molecular Biology
KW  - bisacsh
KW  - fast
KW  - Electronic books
N1  - Includes bibliographical references and index; Note continued: 9.3.P Elements and Hybrid Dysgenesis -- 9.4.P-Element Structure Varies -- 9.5.Transposition Method of P Elements -- 9.6.Origin of P Elements in D. melanogaster -- 9.7.P Vectors and Germ-Line Transformation -- 9.7.1.Protocols -- 9.7.2.Characterizing Transformants -- 9.8.Using P-Element Vectors -- 9.8.1.Transposon Tagging -- 9.8.2.Expressing Exogenous Genes -- 9.8.3.Evaluating Position Effects -- 9.8.4.Targeted Gene Transfer -- 9.9.Transformation of Other Insects with P Vectors -- 9.10.Evolution of Resistance to P Elements -- 9.11.Using P to Drive Genes into Populations -- 9.12.Relationship of P to Other Transposable Elements (TEs) -- 9.13.Other TEs Can Transform D. melanogaster -- 9.14.Improved Transformation Tools for Drosophila -- 9.15.TE Vectors to Transform Insects other than Drosophila -- 9.15.1.piggyBac -- 9.15.2.Hermes and Herves -- 9.15.3.Minos -- 9.15.4.mariner -- 9.15.5.hobo -- 9.16.Cross Mobilization of TE Vectors -- 9.17.Conversion of Inactive TE Vectors to Activity -- 9.18.Suppression of Transgene Expression -- 9.19.Other Transformation Methods -- 9.19.1.JcDNV Gene Vectors for Somatic Transformations v -- 9.19.2.RNAi for Drosophila -- 9.19.3.Zinc-Finger Nucleases (ZFNs) -- 9.19.4.Transcription Activator-Like Effector Nucleases (TALENs) -- 9.19.5.Meganucleases (or Homing Endonucleases) -- 9.19.6.Cell-Penetrating Peptides -- 9.19.7.Nanotechnology Approaches -- 9.20.Conclusions -- General References -- References Cited -- pt. III APPLICATIONS IN ENTOMOLOGY -- ch. 10 Sex Determination in Insects -- 10.1.Overview -- 10.2.Introduction -- 10.3.Costs and Benefits of Sexual Reproduction -- 10.3.1.Sexual Reproduction Has Costs -- 10.3.2.Advantages of Sex Must Be Large -- 10.3.3.Origin of Sex -- 10.4.Sex Determination Involves Soma and Germ-Line Tissues -- 10.5.Sex Determination in Drosophila melanogaster -- 10.5.1.Dosage Compensation of X Chromosomes -- 10.5.2.Somatic-Sex Determination -- 10.5.3.Germ-Line Determination -- 10.6.Are Sex-Determination Mechanisms Diverse? -- 10.6.1.Intraspecific Variability -- 10.6.2.Environmental Effects -- 10.6.3.Postzygotic Sex Determination -- 10.7.A Single Model? -- 10.8.Meiotic Drive Can Distort Sex Ratios -- 10.8.1.Segregation Distorter (SD) -- 10.8.2.Distorter in Mosquitoes -- 10.8.3.Female-Biased Sex Ratios in Stalk-Eyed Flies -- 10.8.4.Meiotic Drive as a Pest-Management Tool? -- 10.9.Hybrid Sterility -- 10.10.Medea in Tribolium -- 10.11.Cytoplasmic Agents Distort Normal Sex Ratios -- 10.11.1.Spiroplasma Strains -- 10.11.2.L-Form Bacteria -- 10.11.3.Rickettsia -- 10.11.4.Wolbachia -- 10.11.5.Cardinium -- 10.12.Paternal Sex-Ratio Chromosomes and Cytoplasmic Incompatibility in Nasonia -- 10.13.Male Killing in the Coccinellidae -- 10.14.Sex and the Sorted Insects -- 10.14.1.Genetic Control -- 10.14.2.Genetic Improvement of Parasitoids -- 10.15.Conclusion -- References Cited -- ch. 11 Molecular Genetics of Insect Behavior -- 11.1.Overview -- 11.2.Introduction -- 11.3.The Insect Nervous System -- 11.4.Traditional Genetic Analyses of Behavior -- 11.4.1.Crossing Experiments -- 11.4.2.Selection Experiments -- 11.4.3.Some Polygenically Determined Behaviors -- 11.5.Molecular-Genetic Analyses of Insect Behavior -- 11.5.1.The Photoperiodic Clock -- 11.5.2.Learning in Drosophila -- 11.5.3.Functional Genomics of Odor Behavior in Drosophila -- 11.5.4.Behavior of Apis mellifera -- 11.5.5.Pheromones in Insects -- 11.5.6.Neurobiochemistry of Drosophila -- 11.5.7.Divergent Functions of Est-6 and Est-5 in Two Drosophila Species: A Cautionary Tale of Homologs -- 11.5.8.Courtship Behavior in Drosophila -- 11.5.9.Speciation Genes in Drosophila and Other Insects -- 11.5.10.Personality in Insects: Tribolium confusum, Apis mellifera, Acyrthosiphon pisum, and Pyrrhocoris apterus -- 11.6.Symbionts and Insect Behavior -- 11.7.Human Neurodegenerative Diseases and Addictions in Drosophila -- 11.8.High-Throughput Ethomics -- 11.9.Systems Genetics of Complex Traits in Drosophila -- 11.10.Social Behavior in Bees and Ants -- 11.11.Conclusions -- References Cited -- ch. 12 Molecular Systematics and the Evolution of Arthropods -- 12.1.Overview -- 12.2.Introduction -- 12.3.Controversies in Molecular Systematics and Evolution -- 12.3.1.Molecular versus Morphological Traits -- 12.3.2.The Molecular Clock -- 12.3.3.The Neutral (or Nearly Neutral) Theory of Evolution -- 12.3.4.Homology and Similarity -- 12.4.Molecular Methods for Molecular Systematics and Evolution -- 12.4.1.Protein Electrophoresis -- 12.4.2.Molecular Cytology -- 12.4.3.Restriction Fragment Length Polymorphism (RFLP) Analysis -- 12.4.4.DNA and Genome Sequencing -- 12.4.5.Fragment Analyses of Genomic DNA -- 12.5.Targets of DNA Analysis -- 12.5.1.Mitochondria -- 12.5.2.Ribosomal RNA -- 12.5.3.Satellite DNA -- 12.5.4.Introns -- 12.5.5.Nuclear Protein-Coding Genes -- 12.5.6.Rare Genomic Changes -- 12.5.7.MicroRNAs -- 12.6.Steps in Phylogenetic Analysis of DNA Sequence Data -- 12.6.1.Gene Trees or Species Trees -- 12.6.2.Rooted or Unrooted Trees -- 12.6.3.Tree Types -- 12.6.4.Project Goals and Appropriate DNA Sequences -- 12.6.5.Sequence Comparisons with BLAST -- 12.6.6.Aligning Sequences -- 12.6.7.Constructing Phylogenies -- 12.6.8.Artifacts -- 12.6.9.Software Packages -- 12.7.The Universal Tree of Life -- 12.7.1.Two Domains -- 12.7.2.Three Domains -- 12.7.3.Origin of Eukaryota -- 12.8.The Fossil Record of Arthropods -- 12.9.Molecular Analyses of Arthropod Phylogeny -- 12.9.1.Evolution of the Ecdysozoa -- 12.9.2.Relationships among the Arthropoda -- 12.9.3.The Phylogeny of the Holometabola -- 12.9.4.Congruence Between Morphology- and Molecular-Based Trees -- 12.9.5.Genomes and Arthropod Phylogenies -- 12.10.Molecular Evolution and Speciation -- 12.10.1.Species Concepts -- 12.10.2.How Many Genes are Involved in Speciation? -- 12.10.3.Detecting Cryptic Species -- 12.11.Some Conclusions -- Relevant Journals -- References Cited -- ch. 13 Insect Population Ecology and Molecular Genetics -- 13.1.Overview -- 13.2.Introduction -- 13.3.What is Molecular Ecology? -- 13.4.Collecting Arthropods in the Field for Analysis -- 13.5.Molecular Ecological Methods -- 13.5.1.Allele-Specific PCR -- 13.5.2.Allozymes (Protein Electrophoresis) -- 13.5.3.Amplified Fragment Length Polymorphisms (AFLP-PCR) -- 13.5.4.Double-Strand Conformation Polymorphism (DSCP) -- 13.5.5.Heteroduplex Analysis (HDA) -- 13.5.6.Microarrays -- 13.5.7.Microsatellites -- 13.5.8.RFLP Analysis -- 13.5.9.PCR-RFLP -- 13.5.10.RAPD-PCR -- 13.5.11.Sequencing -- 13.5.12.Single Nucleotide Polymorphism (SNP) Markers -- 13.6.Analysis of Molecular Data -- 13.6.1.Allozymes -- 13.6.2.Microsatellites -- 13.6.3.RAPD-PCR -- 13.6.4.RFLPs -- 13.6.5.Sequencing -- 13.7.Case Studies in Molecular Ecology and Population Biology -- 13.7.1.Genetic Variability in the Fall Army worm: Incipient Species or Multiple Species? -- 13.7.2.Analyses of Natural Enemies -- 13.7.3.Population Isolation and Introgression in Periodical Cicadas -- 13.7.4.Eradicating Medflies in California? -- 13.7.5.Plant Defenses to Insect Herbivory -- 13.7.6.Origins of Insect Populations -- 13.8.Applied Pest Management -- 13.8.1.Monitoring Biotypes, Species, and Cryptic Species -- 13.8.2.Monitoring Vectors of Disease -- 13.8.3.Pesticide Resistances and Pest Management -- 13.8.4.Monitoring Pest-Population Biology -- 13.8.5.The "So What?" Test -- Relevant Journals -- References Cited -- ch. 14 Genetic Modification of Pest and Beneficial Insects for Pest-Management Programs -- 14.1.Overview -- 14.2.Introduction -- 14.3.Why Genetically Modify Insects? -- 14.3.1.Beneficial Insects -- 14.3.2.Pest Insects -- 14.4.Why Use Molecular-Genetic Methods? -- 14.5.What Genetic Modification Methods are Available? -- 14.5.1.Transposable-Element (TE) Vectors and Transgenesis -- 14.5.2.Paratransgenesis (Genetic Modification of Symbionts) -- 14.5.3.Viral Vectors -- 14.5.4.Transfer of Wolbachia from Another Arthropod -- 14.5.5.Site-Specific Modifications -- 14.5.6.No Vectors -- 14.5.7.RNAi to Control Pests -- 14.6.Methods to Deliver Exogenous Nucleic Acids into Arthropod Tissues -- 14.7.What Genes are Available? -- 14.8.Why are Regulatory Signals Important? -- 14.9.How are Modified Arthropods Identified? -- 14.10.How to Deploy Genetically Modified Pest and Beneficial Arthropods -- 14.11.Potential Risks Associated with Releases of Genetically Modified Arthropods -- 14.11.1.Could Gene Silencing Reduce Program Effectiveness? -- 14.11.2.Relative Risks -- 14.11.3.General Risk Issues -- 14.11.4.Horizontal Transfer (HT) -- 14.12.Permanent Releases of Genetically Modified Arthropods into the Environment -- 14.12.1.Models to Predict? -- 14.13.Regulatory Issues: Releases of Genetically Modified Arthropods -- 14.14.Conclusions -- References Cited
N2  - This book summarizes and synthesizes two rather disparate disciplines-entomology and molecular genetics. It provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools
UR  - http://148.231.10.114:2048/login?url=http://www.sciencedirect.com/science/book/9780124158740
ER  -