TY  - BOOK
AU  - Aransay,Ana M.
AU  - Lavín Trueba,José Luis
ED  - SpringerLink (Online service)
TI  - Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing
SN  - 9783319313504
AV  - RB155-155.8 
U1  - 611.01816 23
PY  - 2016///
CY  - Cham
PB  - Springer International Publishing, Imprint: Springer
KW  - Medicine
KW  - Human genetics
KW  - Systems biology
KW  - Biostatistics
KW  - Biomedicine
KW  - Human Genetics
KW  - Systems Biology
N1  - The High Throughput Sequencing technologies triple-W discussion: Why to use HTS, What is the optimal HTS method to use, Which data analysis workflow to follow -- Whole genome sequencing recommendations -- Counseling for targeted DNA region re-sequencing -- Transcriptome profiling strategies -- Differential mRNA Alternative Splicing -- microRNA discovery and expression analysis in animals -- Analysis of Long Non-coding RNAs in RNA-seq data -- Ribosome profiling -- Genome-wide analysis of DNA methylation patterns by high-throughput sequencing -- Characterization of DNA-protein interactions: Design and analysis of ChIP-seq experiments -- PAR-CLIP: A genomic technique to dissect RNA-protein interactions -- Metagenomic design and sequencing -- A hitchhiker's guide to Metatranscriptomics -- Eukaryotic Single-Cell mRNA Sequencing -- Eukaryotic Single-Cell DNA Sequencing -- Submitting data to a public repository, the final step of a successful HTS experiment
N2  - High throughput sequencing (HTS) technologies have conquered the genomics and epigenomics worlds. The applications of HTS methods are wide, and can be used to sequence everything from whole or partial genomes, transcriptomes, non-coding RNAs, ribosome profiling, to single-cell sequencing. Having such diversity of alternatives, there is a demand for information by research scientists without experience in HTS that need to choose the most suitable methodology or combination of platforms and to define their experimental designs to achieve their specific objectives. Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing aims to collect in a single volume all aspects that should be taken into account when HTS technologies are being incorporated into a research project and the reasons behind them. Moreover, examples of several successful strategies will be analyzed to make the point of the crucial features. This book will be of use to all scientist that are unfamiliar with HTS and want to incorporate such technologies to their research
UR  - http://148.231.10.114:2048/login?url=http://dx.doi.org/10.1007/978-3-319-31350-4
ER  -