Guidelines for Molecular Analysis in Archive Tissues [recurso electrónico] / edited by Giorgio Stanta.

Por: Stanta, Giorgio [editor.]Colaborador(es): SpringerLink (Online service)Tipo de material: TextoTextoEditor: Berlin, Heidelberg : Springer Berlin Heidelberg, 2011Descripción: XVI, 323p. 46 illus., 31 illus. in color. online resourceTipo de contenido: text Tipo de medio: computer Tipo de portador: online resourceISBN: 9783642178900Tema(s): Medicine | Oncology | Pathology | Medicine & Public Health | Pathology | Molecular Medicine | OncologyFormatos físicos adicionales: Printed edition:: Sin títuloClasificación CDD: 616.07 Clasificación LoC:RB1-214Recursos en línea: Libro electrónicoTexto
Contenidos:
Introduction -- Archive Tissues -- Pre-Analytical Treatment of Tissues -- Dissection of Tissue Components -- Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids -- Reverse Transcription (RT) -- Nucleic Acid Amplification -- DNA Sequencing -- Microsatellite Analysis -- Methylation Analysis -- Analysis of Copy Number Variations -- Microarrays -- Protein Extraction in Archive Tissues -- Protein Electrophoretic Separation -- Protein Immunodetection -- Mass Spectrometry -- Elements of Good Laboratory Practice. -- DNA Sequencing -- Microsatellite Analysis -- Methylation Analysis -- Analysis of Copy Number Variations -- Microarrays -- Protein Extraction in Archive Tissues -- Protein Electrophoretic Separation -- Protein Immunodetection -- Mass Spectrometry -- Elements of Good Laboratory Practice.
En: Springer eBooksResumen: A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care.   This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. The topics addressed include pre-analytical treatment of tissues, isolation of tissue components, DNA and RNA methods, proteomics methods, internal quality control procedures, and storage and treatment of reagents. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.
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Existencias
Tipo de ítem Biblioteca actual Colección Signatura Copia número Estado Fecha de vencimiento Código de barras
Libro Electrónico Biblioteca Electrónica
Colección de Libros Electrónicos RB1 -214 (Browse shelf(Abre debajo)) 1 No para préstamo 375614-2001

Introduction -- Archive Tissues -- Pre-Analytical Treatment of Tissues -- Dissection of Tissue Components -- Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids -- Reverse Transcription (RT) -- Nucleic Acid Amplification -- DNA Sequencing -- Microsatellite Analysis -- Methylation Analysis -- Analysis of Copy Number Variations -- Microarrays -- Protein Extraction in Archive Tissues -- Protein Electrophoretic Separation -- Protein Immunodetection -- Mass Spectrometry -- Elements of Good Laboratory Practice. -- DNA Sequencing -- Microsatellite Analysis -- Methylation Analysis -- Analysis of Copy Number Variations -- Microarrays -- Protein Extraction in Archive Tissues -- Protein Electrophoretic Separation -- Protein Immunodetection -- Mass Spectrometry -- Elements of Good Laboratory Practice.

A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care.   This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. The topics addressed include pre-analytical treatment of tissues, isolation of tissue components, DNA and RNA methods, proteomics methods, internal quality control procedures, and storage and treatment of reagents. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.

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