The condensed protocols from Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell.

Por: Sambrook, JosephColaborador(es): Russell, David W. (David William), 1957- [coaut.]Tipo de material: TextoTextoDetalles de publicación: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006Descripción: v, 800 p. : il. ; 28 cmISBN: 0879697725 (hardcover : alk. paper); 0879697717 (pbk. : alk. paper); 9780879697716Tema(s): Clonamiento molecular -- Manuales de laboratorio | Molecular cloning -- Laboratory manuals | Cloning, Molecular -- Laboratory ManualsClasificación LoC:QH442.2 | S352 2006Recursos en línea: Table of contentsTexto
Contenidos:
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
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Libro Libro Biblioteca Central Mexicali
Acervo General QH442.2 S352 2006 (Browse shelf(Abre debajo)) 1 Disponible MXL104438

"A single-volume adaptation of the three-volume third edition of Molecular cloning: a laboratory manual"--P. [4] of cover.

Incluye referencias bibliográficas e índice.

Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.

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