000 02039cam a2200361 a 4500
999 _c138898
_d138898
001 u294126
003 MX-MeUAM
005 20220810102004.0
008 971209s1998 nyua fr 001 0 eng c
020 _a0471165727 (pbk. alk. paper)
040 _aDNLM/DLC
_cDLC
_dNLM
_dC#P
_dVAM
_dUKM
_dBAKER
050 4 _aQH442.6
_b.D53 1998
245 0 0 _aDNA transfer to cultured cells /
_cedited by Katya Ravid, R. Ian Freshney.
260 _aNew York :
_bWiley-Liss,
_cc1998.
300 _axvi, 296 p. :
_bil. ;
_c26 cm.
490 0 _aCulture of specialized cells
504 _aIncluye referencias bibliográficas e índice.
505 0 _aCh. 1. Production and use of retroviruses-- Ch. 2. Baculovirus expression and the study of the regulation of the tumor suppressor protein p53-- Ch. 3. Electroporation of DNA into cultured cell lines-- Ch. 4. DNA transfer to blastula-derived cultures from ZebraFish-- Ch. 5. Transfection of rat adipose cells by electroporation-- Ch. 6. Calcium phosphate transfection-- Ch. 7. Optimization of calcium phosphate transfection for reporter gene analysis-- Ch. 8. Strontium phosphate transfection.
505 0 _aCh. 9. In vitro tissue transfection by calcium phosphate-- Ch. 10. DEAE-Dextran transfection-- Ch. 11. Cationic liposomes-- Ch. 12. Transfection of mammalian cells with yeast artificial chromosomes-- Ch. 13. Mapping human senescence genes using microcell-mediated chromosome transfer-- Ch. 14. Methotrexate and DHFR amplification.
650 7 _aIngeniería genética
_xMetodología.
_2lemb
650 7 _aTransfección.
_2lemb
650 0 _aGenetic engineering
_xMethodology.
650 0 _aTransfection.
650 0 _aCell culture.
650 1 2 _aTransfection.
650 2 2 _aCells, Cultured.
650 2 2 _aDNA
_xgenetics.
700 1 _aRavid, Katya.
700 1 _aFreshney, R. Ian.
856 4 2 _3Publisher description
_uhttp://www.loc.gov/catdir/description/wiley037/97050456.html
856 4 _3Table of Contents
_uhttp://www.loc.gov/catdir/toc/onix05/97050456.html
942 _cLIBRO