000			02193cam a2200325 a 4500
001			u328251
003			SIRSI
005			20160812065623.0
008			110623s2006 nyua fr 001 0 eng c
020			_a0879697725 (hardcover : alk. paper)
020			_a0879697717 (pbk. : alk. paper)
020			_a9780879697716
040			_aDNLM/DLC _cDLC _dBAKER _dC#P _dUBY _dCOO _dBTCTA _dYDXCP _dDLC
050		0	_aQH442.2 _bS352 2006
100	1		_aSambrook, Joseph.
245	1	4	_aThe condensed protocols from Molecular cloning : a laboratory manual / _cJoseph Sambrook, David W. Russell.
260			_aCold Spring Harbor, N.Y. : _bCold Spring Harbor Laboratory Press, _cc2006.
300			_av, 800 p. : _bil. ; _c28 cm.
500			_a"A single-volume adaptation of the three-volume third edition of Molecular cloning: a laboratory manual"--P. [4] of cover.
504			_aIncluye referencias bibliográficas e índice.
505	0		_aPlasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
650		7	_aClonamiento molecular _vManuales de laboratorio. _2lemb
650		0	_aMolecular cloning _vLaboratory manuals.
650		2	_aCloning, Molecular _vLaboratory Manuals.
700	1		_aRussell, David W. _q(David William), _d1957-, _ecoaut.
856	4	1	_3Table of contents _uhttp://www.loc.gov/catdir/toc/ecip0517/2005022077.html
596			_a2
942			_cLIBRO
999			_c169136 _d169136