000			03364nam a22004575i 4500
001			u374955
003			SIRSI
005			20160812084300.0
007			cr nn 008mamaa
008			101125s2011 gw | s |||| 0|eng d
020			_a9783642151750 _9978-3-642-15175-0
040			_cMX-MeUAM
050		4	_aQH201-278.5
082	0	4	_a570.282 _223
100	1		_aDiaspro, Alberto. _eeditor.
245	1	0	_aOptical Fluorescence Microscopy _h[recurso electrónico] : _bFrom the Spectral to the Nano Dimension / _cedited by Alberto Diaspro.
264		1	_aBerlin, Heidelberg : _bSpringer Berlin Heidelberg : _bImprint: Springer, _c2011.
300			_aXII, 244 p. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0		_aFundamentals of Optical Microscopy -- The White Confocal - Continuous Spectral Tuning in Excitation and Emission -- SHG/THG (Second/Third Harmonic Generation) Microscopy -- Role of Scattering and Nonlinear Effects in the Illumination and the Photobleaching Distribution Profiles -- New Analytical Tools for Evaluation of Spherical Aberration in Optical Microscopy -- Improving Image Formation by Pushing the Signal to Noise Ratio -- Site-specific Labeling of Proteins in Living Cells Using Synthetic Fluorescent Dyes -- Imaging Molecular Physiology in Cells Using FRET-based Fluorescent Nanosensors -- Measuring Molecular Dynamics by FRAP, FCS and SPT -- In vitro – in-vivo Fluctuation Spectroscopies -- Interference X-ray Diffraction from Single Muscle Cells Reveals the Molecular Basis of Muscle Braking -- Low Concentration Protein Detection Using Novel SERS Devices -- Near Infrared 3-Dimensional Nonlinear Optical Monitoring of Stem Cell Differentiation -- A Correlative Microscopy: a Combination of Light and Electron Microscopy.
520			_aIn the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.
650		0	_aLife sciences.
650		0	_aBiochemistry.
650		0	_aCytology.
650		0	_aMicroscopy.
650	1	4	_aLife Sciences.
650	2	4	_aBiological Microscopy.
650	2	4	_aCell Biology.
650	2	4	_aBiophysics and Biological Physics.
650	2	4	_aBiochemistry, general.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9783642151743
856	4	0	_zLibro electrónico _uhttp://148.231.10.114:2048/login?url=http://link.springer.com/book/10.1007/978-3-642-15175-0
596			_a19
942			_cLIBRO_ELEC
999			_c202835 _d202835