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020 _a9789048138456
_9978-90-481-3845-6
040 _cMX-MeUAM
050 4 _aRC261-271
082 0 4 _a614.5999
_223
100 1 _aRibatti, Domenico.
_eauthor.
245 1 4 _aThe Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis
_h[recurso electrónico] :
_bThe CAM assay in the study of angiogenesis and metastasis /
_cby Domenico Ribatti.
264 1 _aDordrecht :
_bSpringer Netherlands,
_c2010.
300 _aX, 124 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aChorioallantoic Membrane Vasculature -- Chorioallantoic Membrane in the Study of Angiogenesis, Antiangiogenesis, and the Vascularization of Grafted Tissues -- Chorioallantoic Membrane in the Study of Tumor Angiogenesis -- Chorioallantoic Membrane in the Study of Tumor Metastasis -- Other Applications of Chorioallantoic Membrane -- Different Morphological Techniques and Methods of Quantifying the Angiogenic Response Used in the Study of Vascularization in the Chorioallantoic Membrane -- Advantages and Limitations of Chorioallantoic Membrane in Comparison with Other Classical In Vivo Angiogenesis Assays.
520 _aThe chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been broadly used to study the morpho-functional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of pro-angiogenic and anti-angiogenic natural and synthetic molecules. The CAM is a suitable site for transplanting tissues, which can survive and develop in the CAM by peripheral anastomoses between graft and original CAM vasculature or by new angiogenic vessels grown from the CAM that invade the graft. While the formation of peripheral anastomoses between host and pre-existing donor vessels is the main, and the most common, mechanism involved in the revascularization of embryonic grafts, the growth of CAM-derived vessels into the graft is only stimulated in tumor grafts. The CAM has long been a favored system for the study of tumor angiogenesis and metastasis, because at this stage the chick immunocompetence system is not fully developed and the conditions for rejection have not been established. Tumors remain avascular for 72 h, after which they are penetrated by new blood vessels and begin a phase of rapid growth. Also, delivery of tumor cells onto the CAM allows the fine study of the effects of tumor derived angiogenic growth factors on blood vessel structure and functionality. The CAM may also used to verify the ability to inhibit the growth of capillaries by implanting tumors onto the CAM and by comparing tumor growth and vascularization with or without the administration of an anti-angiogenic molecule. Other studies using the tumor cells/CAM model have focused on the invasion of the chorionic epithelium and the blood vessels by tumor cells. The cells invade the epithelium and the mesenchymal connective tissue below, where they are found in the form of a dense bed of blood vessels, which is a target for intravasation.
650 0 _aMedicine.
650 0 _aOncology.
650 0 _aPathology.
650 0 _aCytology.
650 1 4 _aBiomedicine.
650 2 4 _aCancer Research.
650 2 4 _aOncology.
650 2 4 _aPathology.
650 2 4 _aCell Biology.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9789048138432
856 4 0 _zLibro electrónico
_uhttp://148.231.10.114:2048/login?url=http://link.springer.com/book/10.1007/978-90-481-3845-6
596 _a19
942 _cLIBRO_ELEC
999 _c205608
_d205608