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001 | u379835 | ||
003 | SIRSI | ||
005 | 20160812084617.0 | ||
006 | m o d | ||
007 | cr cnu---unuuu | ||
008 | 141025s2014 ne ob 001 0 eng d | ||
040 |
_aEBLCP _beng _cEBLCP _dOCLCO _dNT _dUAB _dUIU _dNT _dUKMGB _dOCLCF |
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020 |
_a9781908818331 _qelectronic bk. |
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020 |
_a1908818336 _qelectronic bk. |
||
020 | _z9781907568923 (print) | ||
050 | 4 | _aQR82.E6 | |
082 | 0 | 4 |
_a589.95 _223 |
049 | _aTEFA | ||
100 | 1 | _aMoulton, Garner G. | |
245 | 1 | 0 |
_aFed-batch fermentation _h[recurso electrónico] : _ba practical guide to scalable recombinant protein production in Escherichia coli / _cGarner G. Moulton. |
260 |
_aAmsterdam : _bElsevier, _cc2014. |
||
300 | _a1 online resource (xii, 174 p.). | ||
490 | 1 |
_aWoodhead Publishing Series in Biomedicine ; _vno. 42 |
|
588 | 0 | _aPrint version record. | |
504 | _aIncludes bibliographical references (pages 151-164) and index. | ||
505 | 0 | _aIntroduction to fermentation -- Generation of a recombinant Escherichia coli expression system -- Recombinant fed-batch fermentation using Escherichia coli -- Escherichia coli produced recombinant protein : soluble versus insoluble production -- The future of Escherichia coli recombinant fermentation. | |
520 | _aPrimarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. | ||
650 | 0 | _aEscherichia coli. | |
650 | 0 |
_aRecombinant proteins _xSynthesis. |
|
650 | 7 |
_aNATURE / Plants / Mushrooms _2bisacsh |
|
650 | 7 |
_aEscherichia coli. _2fast _0(OCoLC)fst00915160 |
|
650 | 7 |
_aRecombinant proteins _xSynthesis. _2fast _0(OCoLC)fst01091506 |
|
655 | 4 | _aElectronic books. | |
776 | 0 | 8 |
_iPrint version: _aMoulton, G G _tFed-Batch Fermentation : A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli _dBurlington : Elsevier Science,c2014 _z9781907568923 |
830 | 0 |
_aWoodhead Publishing series in biomedicine ; _vno. 42. |
|
856 | 4 | 0 |
_zLibro electrónico _3ScienceDirect _uhttp://148.231.10.114:2048/login?url=http://www.sciencedirect.com/science/book/9781907568923 |
596 | _a19 | ||
942 | _cLIBRO_ELEC | ||
999 |
_c206756 _d206756 |