000 02737cam a2200469Mu 4500
001 u379835
003 SIRSI
005 20160812084617.0
006 m o d
007 cr cnu---unuuu
008 141025s2014 ne ob 001 0 eng d
040 _aEBLCP
_beng
_cEBLCP
_dOCLCO
_dNT
_dUAB
_dUIU
_dNT
_dUKMGB
_dOCLCF
020 _a9781908818331
_qelectronic bk.
020 _a1908818336
_qelectronic bk.
020 _z9781907568923 (print)
050 4 _aQR82.E6
082 0 4 _a589.95
_223
049 _aTEFA
100 1 _aMoulton, Garner G.
245 1 0 _aFed-batch fermentation
_h[recurso electrónico] :
_ba practical guide to scalable recombinant protein production in Escherichia coli /
_cGarner G. Moulton.
260 _aAmsterdam :
_bElsevier,
_cc2014.
300 _a1 online resource (xii, 174 p.).
490 1 _aWoodhead Publishing Series in Biomedicine ;
_vno. 42
588 0 _aPrint version record.
504 _aIncludes bibliographical references (pages 151-164) and index.
505 0 _aIntroduction to fermentation -- Generation of a recombinant Escherichia coli expression system -- Recombinant fed-batch fermentation using Escherichia coli -- Escherichia coli produced recombinant protein : soluble versus insoluble production -- The future of Escherichia coli recombinant fermentation.
520 _aPrimarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision.
650 0 _aEscherichia coli.
650 0 _aRecombinant proteins
_xSynthesis.
650 7 _aNATURE / Plants / Mushrooms
_2bisacsh
650 7 _aEscherichia coli.
_2fast
_0(OCoLC)fst00915160
650 7 _aRecombinant proteins
_xSynthesis.
_2fast
_0(OCoLC)fst01091506
655 4 _aElectronic books.
776 0 8 _iPrint version:
_aMoulton, G G
_tFed-Batch Fermentation : A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli
_dBurlington : Elsevier Science,c2014
_z9781907568923
830 0 _aWoodhead Publishing series in biomedicine ;
_vno. 42.
856 4 0 _zLibro electrónico
_3ScienceDirect
_uhttp://148.231.10.114:2048/login?url=http://www.sciencedirect.com/science/book/9781907568923
596 _a19
942 _cLIBRO_ELEC
999 _c206756
_d206756