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008 160901t20172017nyua b 001 0 eng d
020 _a9781493964192 (hbk. : alk. paper)
020 _a9781493964215
040 _aBTCTA
_beng
_cBTCTA
_erda
_dYDXCP
_dIAL
_dCOD
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050 0 4 _aTK8360.O69
_bO68 2017
245 1 0 _aOptical tweezers :
_bmethods and protocols /
_cedited by Arne Gennerich
260 _aNew York :
_bHumana Press,
_c©2017
300 _axii, 555 p. :
_bil. (algunas col.) ;
_c26 cm.
490 1 _aMethods in molecular biology,
_x1064-3745 ;
_v1486
490 1 _aSpringer protocols
500 _a"Published In: United States, 20 December 2016" --Distributor's website.
504 _aIncluye referencias bibliográficas e índice
505 0 _aIntroduction to optical tweezers -- Exact theory of optical tweezers and its application to absolute calibration -- Beyond the Hookean spring model : direct measurement of optical forces through light momentum changes -- A surface-coupled optical trap with 1-bp precision via active stabilization -- Implementation and tuning of an optical tweezers force-clamp feedback system -- Custom-made microspheres for optical tweezers -- Optical torque wrench design and calibration -- High-resolution "fleezers" : dual-trap optical tweezers combined with single-molecule fluorescence detection -- Versatile quadruple-trap optical tweezers for dual DNA experiments -- Probing DNA-DNA interactions with a combination of quadruple-trap optical tweezers and microfluidics -- Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement -- Mechanically watching the ClpXP proteolytic machinery -- Deciphering the molecular mechanism of the bacteriophage [phi]29 DNA packaging motor -- Single-molecule protein folding experiments using high-precision optical tweezers -- Observing single RNA polymerase molecules down to base-pair resolution -- Optical tweezers-based measurements of forces and dynamics at microtubule ends -- Simultaneous manipulation and super-resolution fluorescence imaging of individual kinetochores coupled to microtubule tips -- Measurement of force-dependent release rates of cytoskeletal motors -- Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers -- Quantifying force and viscoelasticity inside living cells using an active-passive calibrated optical trap -- Measuring molecular forces using calibrated optical tweezers in living cells.
650 0 _aOptical tweezers
_vLaboratory manuals.
650 1 2 _aOptical Tweezers.
650 2 2 _aBiophysical Phenomena.
650 2 2 _aInvestigative Techniques.
650 4 _a Pinzas ópticas
_vManuales de laboratorio
700 1 _aGennerich, Arne,
_eed.
942 _cLIBRO