000 05137nam a22005175i 4500
001 978-3-031-52193-5
003 DE-He213
005 20250516160013.0
007 cr nn 008mamaa
008 240320s2024 sz | s |||| 0|eng d
020 _a9783031521935
_9978-3-031-52193-5
050 4 _aQH301-705
072 7 _aPSA
_2bicssc
072 7 _aSCI086000
_2bisacsh
072 7 _aPSA
_2thema
082 0 4 _a570
_223
245 1 0 _aAdvanced Technologies for Protein Complex Production and Characterization
_h[electronic resource] :
_bVolume II /
_cedited by M. Cristina Vega, Francisco J. Fernández.
250 _a1st ed. 2024.
264 1 _aCham :
_bSpringer Nature Switzerland :
_bImprint: Springer,
_c2024.
300 _aX, 213 p. 50 illus., 45 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aAdvances in Experimental Medicine and Biology,
_x2214-8019 ;
_v1453
505 0 _aChapter 1: Immunoprecipitation methods to isolate messenger ribonucleoprotein complexes (mRNP) -- Chapter 2: Purification of in vivo or in vitro-assembled RNA-protein complexes by RNA centric methods -- Chapter 3: Peptide-based mass spectrometry for protein complexes investigation -- Chapter 4: Probing protein complexes composition, stoichiometry, and interactions by peptide-based mass spectrometry -- Chapter 5: Discovery and examination of linear motif mediated protein-protein complexes -- Chapter 6: Protein-protein binding kinetics by biolayer interferometry -- Chapter 7: Studying Macromolecular Interactions of Cellular Machines by the Combined use of Analytical Ultracentrifugation, Light Scattering, and Fluorescence Spectroscopy Methods -- Chapter 8: The complementarity of nuclear magnetic resonance and native mass spectrometry in probing protein-protein interactions -- Chapter 9: X-ray crystallography for protein complexes -- Chapter 10: Characterizationof biological samples using ultra-short and ultra-bright XFEL pulses -- Chapter 11: Small-angle X-ray scattering for macromolecular complexes -- Chapter 12: Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines -- Chapter 13: Characterization of Complexes and Supramolecular Structures by Electron Microscopy.
520 _aThis edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume. The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular complexes. In here, we focus on characterization of protein complexes in the broadest sense, which is not typically covered in other sources. Moreover, all chapters are carefully written by world renowned scientists and active researchers, making this volume ideal, not only as a reference source, but also as a companion book for the daily laboratory work. This book is aimed for a wide range of scientists, from science students to experienced researchers, working on protein and protein-nucleic acid complexes, who need a thorough understanding of protein production and complex characterization. .
541 _fUABC ;
_cPerpetuidad
650 0 _aLife sciences.
650 1 4 _aLife Sciences.
700 1 _aVega, M. Cristina.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aFernández, Francisco J.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9783031521928
776 0 8 _iPrinted edition:
_z9783031521942
776 0 8 _iPrinted edition:
_z9783031521959
830 0 _aAdvances in Experimental Medicine and Biology,
_x2214-8019 ;
_v1453
856 4 0 _zLibro electrónico
_uhttp://libcon.rec.uabc.mx:2048/login?url=https://doi.org/10.1007/978-3-031-52193-5
912 _aZDB-2-SBL
912 _aZDB-2-SXB
942 _cLIBRO_ELEC
999 _c274554
_d274553