Fed-batch fermentation
Moulton, Garner G.
Fed-batch fermentation a practical guide to scalable recombinant protein production in Escherichia coli / [recurso electrónico] : Garner G. Moulton. - Amsterdam : Elsevier, c2014. - 1 online resource (xii, 174 p.). - Woodhead Publishing Series in Biomedicine ; no. 42 . - Woodhead Publishing series in biomedicine ; no. 42. .
Includes bibliographical references (pages 151-164) and index.
Introduction to fermentation -- Generation of a recombinant Escherichia coli expression system -- Recombinant fed-batch fermentation using Escherichia coli -- Escherichia coli produced recombinant protein : soluble versus insoluble production -- The future of Escherichia coli recombinant fermentation.
Primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision.
9781908818331 1908818336
Escherichia coli.
Recombinant proteins--Synthesis.
NATURE / Plants / Mushrooms
Escherichia coli.
Recombinant proteins--Synthesis.
Electronic books.
QR82.E6
589.95
Fed-batch fermentation a practical guide to scalable recombinant protein production in Escherichia coli / [recurso electrónico] : Garner G. Moulton. - Amsterdam : Elsevier, c2014. - 1 online resource (xii, 174 p.). - Woodhead Publishing Series in Biomedicine ; no. 42 . - Woodhead Publishing series in biomedicine ; no. 42. .
Includes bibliographical references (pages 151-164) and index.
Introduction to fermentation -- Generation of a recombinant Escherichia coli expression system -- Recombinant fed-batch fermentation using Escherichia coli -- Escherichia coli produced recombinant protein : soluble versus insoluble production -- The future of Escherichia coli recombinant fermentation.
Primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision.
9781908818331 1908818336
Escherichia coli.
Recombinant proteins--Synthesis.
NATURE / Plants / Mushrooms
Escherichia coli.
Recombinant proteins--Synthesis.
Electronic books.
QR82.E6
589.95
